The use of antisense oligonucleotides in Xenopus oocytes
نویسندگان
چکیده
منابع مشابه
Characterization of modified antisense oligonucleotides in Xenopus laevis embryos.
A wide variety of modified oligonucleotides have been tested as antisense agents. Each chemical modification produces a distinct profile of potency, toxicity, and specificity. Novel cationic phosphoramidate-modified antisense oligonucleotides have been developed recently that have unique and interesting properties. We compared the relative potency and specificity of a variety of established ant...
متن کاملEffect of antisense oligonucleotides on the expression of hepatocellular bile acid and organic anion uptake systems in Xenopus laevis oocytes.
A Na(+)-dependent bile acid (Na+/taurocholate co-transporting polypeptide; Ntcp) and a Na(+)-independent bromosulphophthalein (BSP)/bile acid uptake system (organic-anion-transporting polypeptide; oatp) have been cloned from rat liver by using functional expression cloning in Xenopus laevis oocytes. To evaluate the extent to which these cloned transporters could account for overall hepatic bile...
متن کاملDefining Synphenotype Groups in Xenopus tropicalis by Use of Antisense Morpholino Oligonucleotides
To identify novel genes involved in early development, and as proof-of-principle of a large-scale reverse genetics approach in a vertebrate embryo, we have carried out an antisense morpholino oligonucleotide (MO) screen in Xenopus tropicalis, in the course of which we have targeted 202 genes expressed during gastrula stages. MOs were designed to complement sequence between -80 and +25 bases of ...
متن کاملAntisense Oligonucleotides: problems with use and solutions
Les oligonucléotides antisens (OAS) montrent un grand potentiel comme outil moléculaire et comme nouvel agent thérapeutique. Cependant, plusieurs problèmes limitent leur utilisation, en particulier leur toxicité, leurs effets indésirables et leur faible pénétration dans les cellules. Chez l'homme, les études cliniques concernant l'utilisation des oligonucléotides phosphorotioates (PS-ODNs) ont ...
متن کامل: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
ذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Methods
سال: 2010
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2009.12.015